FastQCFastQC Report
Thu 28 Apr 2016
ERR1042196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4357836
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105760.2426892613673392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93710.21503792249180556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54590.12526859661538434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA4300.018.530
ACGGACC6100.017.5901648
GACGGAC5900.017.2457627
TATACTG7800.016.8397455
TAACGCC4950.016.8181824
GTACCGT1403.4802724E-815.8571436
TAACGGC2003.6379788E-1215.72499936
AATAACG5300.015.3584912
GGTATCA51050.015.2566121
CTAGTAC2750.014.83
TCGAACG2006.184564E-1114.7999993
TTTAGAC4700.014.563833
ATAACGC6300.014.3888893
ATCGTTT5700.014.28070329
TTAGACT4300.014.1976744
CGTTTAT5750.013.83478331
TCTATAC6700.013.805973
CGCAAGA7550.013.7218552
CGCATCG5800.013.71551813
TAATACT10450.013.631584
Produced by FastQC (version 0.11.5)