FastQCFastQC Report
Thu 28 Apr 2016
ERR1042194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565712
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.14014031419250908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46440.1302404681028642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA3550.020.32394430
TAACGCC5000.019.2400024
CTAACGC1655.4569682E-1116.8181823
GACGGAC4900.016.6122467
GTACTAG2250.016.4444451
ATAACGC4950.016.4444453
CCGGTCG4350.016.16092120
CGTTTAT4500.016.03333331
GTACCGT1403.4797267E-815.8571426
AATAACG4700.015.7446792
ACGGACC5250.015.5047638
ATCGTTT4900.015.47959229
TATACTG6050.015.2892575
GGTATCA32600.014.9815951
TAGACAG6300.014.9761915
CTACACT9500.014.6052634
ATGGTCG4700.014.56382836
TGCGACG1803.3378456E-914.38888922
CGCATCG5150.014.36893313
TACCGTC3350.014.3582097
Produced by FastQC (version 0.11.5)