FastQCFastQC Report
Thu 28 Apr 2016
ERR1042193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008487
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT229110.38131063610522914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206180.3431479505572701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119270.19850255147427298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.10886268040523346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91750.016.8768391
GTACTAG4750.015.9684211
GTATTAG14600.015.8390421
TAACGCC4750.015.5789474
TCTATAC9700.014.3041243
TATACTG13000.014.2307695
ATAACGC5350.013.8317763
ATACCGC6050.013.45454527
TAACGGC2800.013.21428636
CTAATAC15000.013.0733333
CGTAACG1000.001834048812.94999923
ACGGACC5900.012.8559328
TAACACT12250.012.8367354
AATCGTC3900.012.80769328
GTATCAA121700.012.6626952
CTAATCG4100.012.63414626
TAACGCT2951.8189894E-1212.5423734
TTAACGG2951.8189894E-1212.54237335
ATAATAC13900.012.3776983
TCTAACG1807.477847E-712.3333322
Produced by FastQC (version 0.11.5)