FastQCFastQC Report
Thu 28 Apr 2016
ERR1042192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6471193
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT128020.1978306009417429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117000.18080128347276925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71940.1111696096840258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.017.1856141
ACGGACC6900.016.0869568
GACGGAC7050.015.4822697
GTATTAG17650.015.3031161
TTAACGG3950.014.98734235
TATACTG11050.014.7330325
GCGTTAG1152.212862E-514.4782611
TATATCG5000.014.0599995
TAACGGC4100.013.98780436
ATAACGC8550.013.8479523
ATACCGT6050.013.760336
CTAGCGG4050.013.70370429
TCTATAC10850.013.4700463
TAGACCG1805.171205E-813.3611115
TACACTG21800.013.3233955
TAACGCC5700.013.3070174
CTACACT19050.013.30446154
TACGACG4450.013.3033715
CGGACCA8700.013.1839089
CGTATTA5350.013.14018715
Produced by FastQC (version 0.11.5)