FastQCFastQC Report
Thu 28 Apr 2016
ERR1042191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425450
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175460.5122246712110817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167030.4876147659431607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.19244186895152463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56510.16497102570465194No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38730.11306543665795736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63800.019.166931
TAGACCG609.2419056E-418.55
ACGGACC9300.017.70438
CGCAAGA10100.017.5841582
GACGGAC9400.017.5159597
CGTCGTA3400.017.41176410
AAGACGG10900.016.8027525
CCGTCGT3650.016.7260279
TACCGTC4050.016.4444457
GCGCAAG11000.015.9772721
CGGACCA10450.015.9330169
CAAGACG11750.015.9021264
GTATCAA77350.015.7614742
AGACGGA11600.015.4698286
CTAGGAC5050.015.0198013
ACCGTCG3850.014.8961058
GCGAAAG10850.014.83410218
TCGAACG2006.184564E-1114.83
GTACGAT1152.2122598E-514.47826120
CGAAAGC11650.014.450644519
Produced by FastQC (version 0.11.5)