FastQCFastQC Report
Thu 28 Apr 2016
ERR1042190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419879
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103650.7299917809897886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.7061165071108172No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38500.27114986558713805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27880.19635475980699763No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27030.19036833420312577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT2350.022.8297888
TCGCGTA2350.022.8297889
GGTATCA30550.020.8919811
TTACTCG555.143914E-420.18181819
GACGGAC5450.020.0275237
CGCAAGA5800.019.7758622
GCGCAAG6000.019.1166671
ACGGACC5900.018.58
AAGACGG6050.018.3471075
CGCGTAA2950.018.18644110
CGGACCA6150.018.048789
TACACCG1451.546141E-1017.8620685
AGACGGA6450.017.7829466
CTAGACT1156.408118E-817.6956524
CGAAAGC6250.017.46419
GTATACT1705.456968E-1217.4117644
GCGTAAC3400.017.41176411
GTATCAA36850.017.370422
GGTCGCG3200.017.343757
ATTGACG2150.017.20930332
Produced by FastQC (version 0.11.5)