FastQCFastQC Report
Thu 28 Apr 2016
ERR1042189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7407658
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136460.18421476801439807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133320.17997591141491684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75610.102070046970311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC11450.019.0655028
GACGGAC12050.018.1161827
TCGTTTA8200.016.69512230
GGTATCA69800.015.9025791
CGGACCA14200.015.7640849
TAACGCC10350.015.1932354
AGACGGA15800.015.104436
TATACTG15650.015.012785
ATAACGC11400.014.9298243
AAGACGG17450.014.8424075
TATACCG4000.014.85
ACGACGG7850.014.6114656
TTAGACT8000.014.568754
CGCAAGA16000.014.3375012
TACGACG8300.014.265065
CTAGTAC5200.014.2307693
CGACGGT7950.013.9622637
CGTTTAT10100.013.92079231
GTACTAG5900.013.796611
TCTATAC11350.013.5286343
Produced by FastQC (version 0.11.5)