FastQCFastQC Report
Thu 28 Apr 2016
ERR1042188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907695
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78580.4119107089969833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.33700355664820636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.1740320124548211No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27650.14493931157758447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19440.10190308199161816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC5850.017.7094028
CAAGACG6800.017.4117644
GGTATCA33450.017.3662191
GACGGAC5900.017.2457627
TACCGTC1554.0199666E-1016.7096797
CGTGCGA904.448431E-516.44444510
TATACTA2150.016.3488375
GGTTATC3350.016.0149252
CGAACGT1403.4766344E-815.8571434
GCGGGTA1659.749783E-1015.6969723
GCGCAAG6800.015.5073531
CGCAAGA6700.015.4626872
GTATCAA37250.015.3959732
AGACGGA6750.015.3481486
GTATACT2900.015.3103464
GCGAAAG6550.015.25190818
TAACACG855.366455E-415.2352944
CGGACCA6650.015.0225569
AAGACGG8000.014.85
GTACACT2900.014.6724154
Produced by FastQC (version 0.11.5)