FastQCFastQC Report
Thu 28 Apr 2016
ERR1042187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379264
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115350.21443453974372703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115240.21423005080248897No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72020.13388448679968115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70920.13183959738730056No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA59600.11079582634352952No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC58440.10863939750865546No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG57210.10635283934753899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC14200.019.6725358
GACGGAC14250.019.343867
TTAACGG2950.018.81355935
AAGACGG16200.018.55
CGCAAGA14950.018.314382
TCGTTTA10300.017.6019430
CGGACCA15500.017.545169
TACGACG11250.017.4311125
CTAGCGG7600.017.28289429
GGTATCA63850.016.718091
ACGACGG11850.016.3924056
AGACGGA16600.016.382536
CGTTTAT11400.016.06578831
TCTAGCG8400.015.85714428
CTAGTAC4250.015.6705873
GTACTAG3600.015.4166661
CGAACGA6850.015.3941616
GCGAAAG17600.015.34659118
ATGGTCG11100.015.33333336
GACGGTA12200.015.3155748
Produced by FastQC (version 0.11.5)