FastQCFastQC Report
Thu 28 Apr 2016
ERR1042186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646524
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105661.6342780778439778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100661.5569414283151128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.7203135537118498No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG38520.595801547970377No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA27320.4225674530257191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20020.309655944713576No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18980.293569921611572No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG17700.2737717393321826No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16510.2553656167443127No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14760.2282977894092099No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG14470.2238122637365357No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10990.16998595566444555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.15962284462757761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10260.15869480483323126No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.14709430740390148No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.12837883821791612No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG8240.12745079842356974No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.12497602563864606No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8020.12404798584429967No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7280.11260216171402762No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7030.10873532923758437No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6790.10502317006019886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT250.005494586729.68
CGCAAGA751.4551915E-1129.62
CGGACCA851.8189894E-1228.2941199
CGAACGA604.3166438E-827.75000216
ACGGACC803.274181E-1127.758
GACGGAC856.91216E-1126.1176477
ACGAACG659.3785275E-825.61538515
CGTGAAA802.7212081E-823.12510
ACCGTGA802.7212081E-823.1258
TAACGAA753.7355312E-722.213
GCGCAAG1059.804353E-1021.1428571
GTTATTC705.096228E-621.1428573
CGATAAC806.950431E-720.812510
AACCGCG450.003824024720.5555557
GTATATC450.003824024720.5555554
CGATGCG450.003824024720.55555533
TACCCCG555.1397487E-420.1818185
CTCCCGC555.1397487E-420.18181824
GCGTTAT759.255487E-619.7333341
CCGATAA851.243583E-619.5882369
Produced by FastQC (version 0.11.5)