FastQCFastQC Report
Thu 28 Apr 2016
ERR1042185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773946
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100230.2655840862587859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT92900.246161444811346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41460.10985848764131761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC5200.018.144238
TAGACCG953.610805E-617.5263165
GACGGAC6000.016.657
TATATCG2350.016.5319165
CGAACGA3250.016.50769216
CGCAAGA5950.016.478992
GGTATCA50500.016.2653471
CGAACGT1059.354329E-615.8571444
CGGACCA6150.015.3414639
GTATACT5200.015.2980774
TTAGACT4000.014.84
TACCGAC1904.5656634E-1014.6052647
AAGACGG8300.014.4879525
ATTGACG5500.014.46363532
TCCGATA3850.014.41558558
TATACTG6900.014.2101445
TCGTTTA3300.014.01515130
AATCGTC2800.013.87500128
CTAATCG2800.013.87500126
ACGAACG4050.013.70370415
Produced by FastQC (version 0.11.5)