FastQCFastQC Report
Thu 28 Apr 2016
ERR1042184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469534
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT75350.2171761395046136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73850.21285279233464782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71970.2074341972149574No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT56520.16290372136431003No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC55720.16059793620699495No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG54620.15742748161568673No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA53530.15428584933884493No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC47140.13586839039479076No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC46370.13364907218087502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45740.1318332663694894No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC42920.12370537368995375No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA42610.12281188194149416No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC41530.1196990719791188No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA39500.11384814214243182No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC39260.11315640659523729No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG39010.11243584873357633No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC37490.10805485693467767No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG37420.10785310073341262No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA37060.10681549741262082No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG36100.10404855522384274No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT35850.10332799736218178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG14900.021.2315435
GACGGAC14950.020.5418077
ACGGACC15600.019.9230778
CAAGACG17000.019.697064
TCTAGCG8650.019.4624328
CTAGCGG8700.019.35057629
CGCAAGA16150.019.2445832
TAAACGC10350.019.12560528
AGACGGA16350.019.0091746
CGGACCA16700.018.7215589
GTAAACG10500.018.6761927
GCGCAAG18100.017.988951
GCAAGAC19750.017.891143
TTCGGGC11700.017.55128335
ACTCTAA12550.017.54183210
TACCGTC10100.017.4009917
TAGACCG752.0687828E-417.2666665
GTTCAAA16300.017.1380371
CGGTCCA10200.016.86764710
CGAGCCG15300.016.8071915
Produced by FastQC (version 0.11.5)