FastQCFastQC Report
Thu 28 Apr 2016
ERR1042183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957478
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC47540.2428635213269319No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA43760.2235529594713197No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC38700.19770337137888652No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37870.19346322155344786No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT34080.17410157355536052No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG33800.17267116156605591No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC33320.170219026727248No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC26570.135735880556512No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA25780.131700075300974No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC25740.13149573073107335No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC25280.1291457681772158No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC24610.12572299663137979No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA22690.11591445727614819No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG22140.11310471944001414No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC20840.10646352091824277No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC20820.10636134863329244No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG20630.10539071192626431No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG20370.10406247222191002No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG20090.10263206023260543No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20040.10237662952022959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.024.376471
AAGACGG7250.023.9862085
CAAGACG8050.022.521744
GACGGAC7450.021.6040277
TTAACGG603.7280108E-521.58333235
ACGGACC7900.020.8417728
CGCAAGA8350.020.604792
CGGACCA8750.019.451439
AGACGGA8450.019.2662736
GCGCAAG9600.018.8854161
CTTCGGG6750.018.63703734
GCAAGAC10650.018.0657273
GTATCAA11550.017.779222
CGAACGA4500.017.67777816
TTCGGGC6650.017.52631635
CCGAACT1502.5102054E-1017.26666623
GTTCAAA8900.017.252811
GCTTCGG7100.017.19718433
GAGCGAA9500.017.13684316
ACGCTTC7250.017.09655231
Produced by FastQC (version 0.11.5)