FastQCFastQC Report
Thu 28 Apr 2016
ERR1042182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571641
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37450.2382859698875252No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32510.20685385530156059No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28580.18184814470989238No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG26850.1708405418285728No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT26290.16727738713866588No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC22850.14538943690066625No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22470.1429715819325151No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA21150.13457271730630596No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC20810.13240937338743392No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC19920.1267465025409747No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC19100.12152902603075386No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG19040.12114725945683523No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18410.11713871043068996No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG17790.11319378916686444No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG17760.11300290587990514No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT17560.11173035063350982No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA17450.11103044524799238No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA16810.10695826845952734No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA16500.10498580782761459No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG16270.10352236929425994No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG16190.10301334719570181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG7750.023.8709665
CAAGACG8200.023.0121944
CGCAAGA8050.022.7515532
ACGGACC8350.021.9341328
GACGGAC8350.021.9341327
GCGCAAG8550.021.4210531
GCAAGAC9350.020.9732633
AGACGGA8850.020.4858766
CGGACCA9100.020.1263739
TCGTTTA4150.019.61445830
TCGAACG2650.019.5471693
CGAACGT2650.018.8490544
GTATACG609.238874E-418.51
TCTAGCG3450.018.23188428
TTAGAGT2650.018.1509424
TATCTAG4500.018.0888881
CGTTTAT4550.017.8901131
TTATCTA2400.017.7291684
CTAGCGG3600.017.47222329
TAGAGTG2700.017.129635
Produced by FastQC (version 0.11.5)