FastQCFastQC Report
Thu 28 Apr 2016
ERR1042181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11533619
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT225580.19558475097885583No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG154860.1342683506365175No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC151470.1313291170793833No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC142770.12378595131328685No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT136660.11848839466606276No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA130130.11282668518875125No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG120940.10485867445421944No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT120520.10449452162413203No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC120180.10419973123787078No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA119710.103792226880392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG46250.020.845
CAAGACG50150.019.293124
GACGGAC49250.018.6690357
ACGGACC49250.018.5939088
CGCAAGA51850.017.9826412
TCGTTTA30800.017.95941530
AGACGGA54100.017.5425156
CGGACCA53350.017.3730099
GGTTATC25700.017.2762642
GCGCAAG54350.017.0533581
GGTATCA71100.017.0168761
CGTTTAT34050.016.57121831
GTAAACG31850.016.49607727
TAACGGC4800.016.187536
TCTAGCG20100.016.10696428
TAAACGC32400.016.04475228
GCAAGAC65250.016.0191573
CGAACGA18400.015.98641316
ACGAACG18500.015.915
AGGTTAT27600.015.8858691
Produced by FastQC (version 0.11.5)