FastQCFastQC Report
Thu 28 Apr 2016
ERR1042180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768698
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC78040.28186533887047266No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC75800.27377489346978257No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA56030.20236948919672712No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC48660.17575047910606356No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC41900.1513346706646951No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT36290.13107243910314523No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG35740.1290859458127972No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC35740.1290859458127972No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG34410.12428224385613743No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC34240.12366823683912077No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG32900.11882841682263649No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT30730.11099079784071791No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT29610.10694557514037285No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC29460.10640380424300519No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA29400.10618709588405813No Hit
TCACTGTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATC28860.10423672065353463No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC28730.103767185875816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.020.5555551
TATACCG1202.401066E-1020.0416685
ATTAGAG4850.017.164953
GTTCAAA11500.016.8913041
AGTATGG11150.016.7578472
GTATGGT11000.016.6500023
ATTGACG10450.016.64114832
ACGGACC7600.016.5526318
GCGGGTA6200.016.4112923
TCGTTTA6550.016.3816830
TTGACGG10850.016.36866433
GAGTATG12250.016.3102041
AAGACGG8300.016.2710845
CGAACGA4450.016.21348216
TACCGCA10750.016.17674428
GACGGAC7600.016.065797
ATAATAC2200.015.9772733
TTATACC2550.015.9607844
ATACCGT6050.015.9008266
AACCCGT8850.015.88700529
Produced by FastQC (version 0.11.5)