FastQCFastQC Report
Thu 28 Apr 2016
ERR1042179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5483287
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT119550.21802615839732628No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG80740.14724744482643348No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC78360.1429069826182726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76170.1389130278973178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73290.1336607038807197No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA64570.11775783394157556No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC62230.11349032067808963No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG59530.10856626691252894No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT58790.10721671143604193No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC55520.10125313520886285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG23650.022.0591965
CAAGACG25000.020.7944
CGCAAGA25950.020.1040482
ACGGACC25300.019.7430828
GACGGAC25200.019.601197
GCGCAAG26750.019.4336451
CGGACCA27150.018.806639
CGTATAC1205.185939E-918.53
GGTTATC11250.018.2533342
AGACGGA27600.018.2318846
TCGTTTA15100.017.76490230
CGAACGA10250.017.68780516
TATCCCG2000.017.5749995
ACGAACG10550.017.18483415
GCAAGAC34200.016.8771933
TAACGAA10750.016.86511613
ATGGTCG14900.016.76174536
TTAACGG3550.016.67605635
CGTTTAT16000.016.6531
TAACGCC16800.016.6279754
Produced by FastQC (version 0.11.5)