FastQCFastQC Report
Thu 28 Apr 2016
ERR1042177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974608
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT89390.22490268222677556No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC76320.1920189362070423No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA72470.18233244636955392No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT67850.17070865856456788No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG65040.16363877896889456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62540.157348850502993No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC59520.14975061691618394No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC57380.14436643814937222No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC54400.13686884341801758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.13334648347711273No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC52500.13208849778393242No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA50410.12683011758643872No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC47790.1202382725541739No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG47660.119911196273947No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG47420.11930736314122047No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG46430.11681655146872345No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT46410.11676623204099626No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG45520.11452701750713529No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA45240.11382254551895432No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA45130.11354578866645465No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG43910.1104763035750947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43700.10994794958395897No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC42120.1059727147935092No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC41190.10363286140419381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.022.5089451
AAGACGG15150.020.392745
GACGGAC15150.019.9042917
CAAGACG16300.019.4079744
ACGGACC15700.019.2070068
TCGTTTA11150.018.91479930
AGACGGA16450.018.7811576
CGCAAGA16700.018.6107792
GCGCAAG17200.018.2848831
TCTAGCG9650.018.21243528
TTAACGG1552.1827873E-1117.90322735
CGGACCA16950.017.8997049
TTCGGGC14100.017.31914735
TAAACGC13800.017.29347828
GTAAACG13850.017.23104727
CTAGCGG10100.017.21782329
CGTTTAT12600.017.17857231
CTTCGGG14950.017.07692334
GTTCAAA21400.016.684581
GCTTCGG15200.016.43092233
Produced by FastQC (version 0.11.5)