FastQCFastQC Report
Thu 28 Apr 2016
ERR1042176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509807
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70090.27926450121463525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.253087189572744No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT53420.21284505143224158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36160.14407482328322457No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC34220.13634514526415775No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG33850.134870928322377No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA30750.12251938097232178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.025.8425731
AAGACGG10750.020.1348825
GTACGAC1153.0559022E-919.3043483
GACGGAC11450.019.2270767
CAAGACG11550.019.2207814
ACGGACC11550.018.7402618
GTATCAA30800.018.740262
TCGAACG2500.018.53
CGCAAGA11650.018.2618032
AGACGGA12100.018.1942166
CGGACCA12100.018.0413239
TCGTTTA5900.017.55932430
CGAACGT2650.017.4528314
TATCTAG9550.017.4345551
CGAAAGC12450.017.23694619
ATTGACG8950.017.15642532
TATACTG4100.017.1463415
GCGCAAG13150.017.0228141
GGTTATC5850.016.7606852
AGCGAAA12950.016.71428517
Produced by FastQC (version 0.11.5)