FastQCFastQC Report
Thu 28 Apr 2016
ERR1042175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065422
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24170.22685846547189753No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23290.21859882750684703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.20555235390296053No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15540.14585769770100487No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14830.13919367161556642No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG14710.13806735734760497No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG10920.1024945983844899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.027.4779421
ACGGACC4250.023.070598
AAGACGG4650.021.4838715
GTATACT1059.822543E-1021.1428574
AGACGGA4700.020.8617026
CGGACCA4700.020.8617029
CGCAAGA4850.020.5979372
ACGAACG1900.020.44736915
GACGGAC4800.020.4270827
TTAGAGT2000.020.3499984
ATAACGA2100.020.26190412
CGTAGAC1101.7535058E-920.1818183
TAACGAA2050.019.8536613
TTCGCGG2250.019.73333432
GTATCAA9600.019.656252
CAAGACG5600.019.4910724
GGTTATC2600.019.2115382
TCGTTTA1950.018.9743630
GCGCAAG5650.018.6637171
TATATCG701.2190909E-418.55
Produced by FastQC (version 0.11.5)