FastQCFastQC Report
Thu 28 Apr 2016
ERR1042174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708419
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73160.27012068664412703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71930.26557929183039997No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT63450.23426951295202111No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA60250.22245450205451964No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC59870.22105146951044136No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG50810.18760021990689033No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT50130.18508953009117127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49530.18287421554788974No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC42190.1557735343017458No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC41380.15278285966831573No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA40750.1504567793978701No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC38070.14056170777121266No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG36460.13461728041340723No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC36390.1343588270500244No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG34740.1282667120560002No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC34720.1281928682378908No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG33640.12420530205998408No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG33470.12357762960605431No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC33240.12272842569779638No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC33110.1222484408800854No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG32640.12051311115451487No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA31840.1175593584301395No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA31600.11667323261282689No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT29900.11039650807352926No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG29140.10759044298537265No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG28110.10378748635273938No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC27280.10072296790119993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10600.021.9905665
CGCAAGA11150.021.0717492
TTAACGG1251.8189894E-1120.7235
GACGGAC11150.020.4080727
ACGGACC11450.020.0349338
CAAGACG12050.019.804984
TCTAGCG7350.019.63265428
TAACGGC1457.2759576E-1219.1379336
CTAGCGG7700.018.7402629
GCGCAAG12350.018.7246951
AGACGGA12400.018.6491956
CGGACCA12500.018.6489
ATTAGAG6400.018.53
TAGAGTG6200.017.9032275
CGTAATA852.7253243E-517.4117642
GGTATCA37800.017.3743381
CGAACGA5650.017.35398316
TTAGAGT6000.017.2666664
TAACGAA5700.017.20175413
ACGAACG5750.017.05217415
Produced by FastQC (version 0.11.5)