FastQCFastQC Report
Thu 28 Apr 2016
ERR1042173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258736
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT134760.14554902526651586No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC125040.13505083199261755No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA110380.11921713719885738No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG103800.11211033557928425No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC100910.10898895918406142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59500.020.9873941
AAGACGG30400.018.6217125
GACGGAC30250.018.0413237
ACGGACC31050.017.6360728
TCGTTTA22500.017.10222230
AGACGGA33850.016.723786
CAAGACG34850.016.4562424
CGAACGA18950.016.1081816
GCGCAAG35100.016.0754991
CGCAAGA35100.016.0227932
ATGGTCG23700.016.00211136
ACGAACG19200.015.99479115
CGTTTAT24250.015.94432931
TAACGCC25500.015.7431364
TGGTCGG24650.015.68559837
GCGGGTA13450.015.5427523
CGGACCA35450.015.3949229
TATCTAG25550.015.3502941
TAACGAA20450.015.28850913
ATAACGC28300.015.2314493
Produced by FastQC (version 0.11.5)