FastQCFastQC Report
Thu 28 Apr 2016
ERR1042172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12966223
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379710.2928454955618147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT374450.2887888014882977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257430.1985389268717652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204080.15739356017554224No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT157870.12175480862854202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG39450.017.1634985
ACGGACC39650.016.563688
GACGGAC38950.016.3863937
CAAGACG41550.015.8062574
TCGTTTA23150.015.5032430
CGCAAGA41450.015.398072
CGGACCA43100.015.3236679
GGTTATC25400.015.0767712
GTATTAG48100.014.9230771
GTAAACG30850.014.81199527
AGACGGA44000.014.7159086
TAAACGC31200.014.58653928
GGTATCA226250.014.3093921
TATCTAG45250.014.0640871
ACGAACG16750.014.02686515
CGAACGA16750.013.91641716
CAAATCG23300.013.81545113
TAGAGTG24700.013.7813765
ATTAGAG25950.013.7591523
GCGCAAG46800.013.756411
Produced by FastQC (version 0.11.5)