FastQCFastQC Report
Thu 28 Apr 2016
ERR1042171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999602
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT311480.44499672981406657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243450.347805489512118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178190.2545716170719421No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118190.1688524576111613No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT86930.12419277553209454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA9150.018.1967216
ACGAACG9350.017.80748615
AAGACGG22650.017.6423845
ACGGACC22300.017.3385648
GACGGAC22350.017.0514557
TCGTTTA13650.016.534830
CGGACCA24350.016.4106779
CGCAAGA23550.016.2611452
CAAGACG25750.015.7339814
AGACGGA24700.015.5789476
ATAACGA10700.015.56074712
CGAACGT6950.015.43884854
GTATTAG24500.015.4040811
TACCGTC14550.015.1305857
CGACGGT15250.014.9213117
TCCGATA11150.014.7668168
GGTTATC13200.014.7159092
TAACGAA11450.014.70305613
ACGACGG15850.014.5899056
CGTTTAT15750.014.5650831
Produced by FastQC (version 0.11.5)