FastQCFastQC Report
Thu 28 Apr 2016
ERR1042170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5479775
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353000.6441870332267292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT233410.42594814568116396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172760.315268418867563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107760.19665040991646557No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT80570.1470315843259988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT57660.10522329840185045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18500.019.85
TAGACCG1254.129106E-1019.245
GACGGAC18750.018.3520017
CAAGACG19800.018.313134
ACGGACC19400.017.8324748
AGACGGA19950.017.5263166
GTATTAG20950.017.219571
CGAACGA7200.016.7013916
TCGTTTA12100.016.66528930
CGGACCA20800.016.6322129
ACGAACG7400.016.515
CGCAAGA21000.016.121432
TCTAGCG10150.015.67487728
CCGGTCG12650.015.64822220
ATTAGAG12300.015.3414633
GCGCAAG22150.015.2844251
CTAGCGG10600.015.18396329
TAACGCC13300.015.16165354
GTAAACG10150.015.12807927
TACCGTC13000.015.0846177
Produced by FastQC (version 0.11.5)