FastQCFastQC Report
Thu 28 Apr 2016
ERR1042169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000960
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155400.38840678237223064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111700.27918299608094055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85650.21407362233064067No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45630.11404762856914341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.10447492601775574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5750.019.30434829
TCTAGCG5900.018.81355928
CGCAAGA13350.017.7378272
ACGGACC13150.017.7262368
AAGACGG13450.017.605955
GACGGAC13150.017.3041827
CAAGACG14400.017.2152774
CGGACCA14250.016.747379
GCGCAAG14700.016.3605441
TATACTG7250.016.3310345
TAGACAG6750.016.170375
AGACGGA14550.015.8934716
GTATTAG13300.015.8571431
TTAACGG2100.015.85714235
TCGTTTA6900.015.81884130
ACGAACG4650.015.51612915
GGTATCA62950.015.3407471
CGGTCCA7400.015.2510
ATTAGAG7650.015.2352943
CGAACGT3050.015.1639354
Produced by FastQC (version 0.11.5)