FastQCFastQC Report
Thu 28 Apr 2016
ERR1042168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5791088
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT245210.42342647875494205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178300.30788687721547314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139510.24090464520656568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73020.12609029598583202No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT64170.11080819355533882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA11550.017.7792230
AAGACGG16950.017.6814175
CCGGTCG11900.017.41176420
GACGGAC16900.016.5295877
ACGGACC17250.016.4086958
TAACGCC12900.016.3488374
AACGCCG13300.016.135345
CAAGACG19150.016.0365524
CGCATCG12850.015.98054513
CGTTTAT13000.015.93846131
ATAACGC15000.015.9099993
AGACGGA18600.015.7150546
ATCGCCG13350.015.65917516
GGTATCA97200.015.6260291
CGGACCA18050.015.5789479
GTAAACG9350.015.03743427
ATGGTCG13050.015.0268236
TAAACGC9500.014.99473728
TGGTCGG13300.014.88345937
ACGCCGC14600.014.698636
Produced by FastQC (version 0.11.5)