FastQCFastQC Report
Thu 28 Apr 2016
ERR1042166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12918883
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224930.17410947989853304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182920.14159118864997847No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT147600.11425136368213877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137000.10604631994886864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC36000.016.238897
ACGGACC36350.016.1843198
TCGTTTA22300.015.59641430
AAGACGG39700.015.4710335
GTATTAG39700.015.2380351
CGAACGT10200.015.2352944
CGGACCA39550.015.2022769
CGCAAGA38350.015.1955682
CAAGACG40500.014.9827174
GGTATCA122650.014.9176521
TATCTAG36100.014.8614961
AGACGGA40950.014.818076
CGAACGA14150.014.38162516
CGACGGT27250.014.256887
ACGAACG14300.014.23076915
TACCGTC23550.014.1401277
GTAAACG20650.014.06537527
TACGACG28050.014.0481285
GGTTATC20750.013.997592
TAACGCC26400.013.9450764
Produced by FastQC (version 0.11.5)