FastQCFastQC Report
Thu 28 Apr 2016
ERR1042165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11674737
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT229840.19686953119372194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT222830.1908651132783548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170940.14641871589912475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.023.1251
TATACTG26400.015.5568185
GTATCAA113600.015.0801052
TACGACG11100.013.8333335
GACGGAC15950.013.686527
ACGACGG10550.013.6777256
AAGACGG20150.013.1290335
TCTATAC22900.012.6834063
CGTCGTA11100.012.66666710
TACACCG10200.012.5147065
CGACGGT11450.012.4410487
AATCGTC6300.012.33333328
CTAATCG6700.012.14925426
TTAGACT13950.012.06814
GACGGTA12000.012.0250018
AGACGGA19700.012.0203056
CCGTCGT11900.011.9705899
ACGGACC17700.011.9152548
TACCGTC12300.011.8821137
TAATCGG7350.011.82993216
Produced by FastQC (version 0.11.5)