FastQCFastQC Report
Thu 28 Apr 2016
ERR1042164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347207
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197610.5903728093302865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181080.5409883523785652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137390.41046161770096684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.18227136833784105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.030.9931771
GTATCAA62550.018.426062
AACACCG2003.6379788E-1215.7249995
GCGTAGA1659.767973E-1015.696971
TATACTG6250.014.2080015
GCGATAC1051.6571244E-414.09523829
TTATACT8050.014.0186344
CGAATAA1907.1395334E-913.63157921
CCGTATA1652.5993904E-713.4545451
TATCAAC95750.013.3702351
ATCAACG97600.013.1547142
TCAACGC99000.012.9500013
GTACGGA2001.4653779E-812.9499996
CGTGCGT1451.3737192E-512.7586210
TCGAATA1901.0477925E-712.65789520
CAACGCA101700.012.6425764
GTATAGG4250.012.6235291
GCTCTAG4550.012.6043971
TACCCGC2501.6007107E-1012.5811
TAAGGTG6950.012.5107915
Produced by FastQC (version 0.11.5)