FastQCFastQC Report
Thu 28 Apr 2016
ERR1042163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3689951
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.11390395157008859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.019.0554281
TATACCG1708.54925E-1116.323535
ACGGACC4850.015.2577328
GACGGAC4550.015.0439567
TCTATAC4800.014.6458343
GTATAAG6600.014.5757571
TATACTG8700.014.459775
CGAACGA1551.2123746E-714.3225816
CTACACT9150.013.7486344
GTATCAA30600.013.6633992
TCTAGCG2750.013.45454628
TTAGACT4000.013.41254
CGGACCA5950.013.3697479
CTAGCGG2800.013.21428629
AAGACGG6500.013.0923085
CTATACT7800.013.0448724
TTATACT9000.012.9499994
GTATTAA6550.012.7099241
GTATTAG8850.012.5423731
TAGTCGG1951.4701254E-712.33333430
Produced by FastQC (version 0.11.5)