FastQCFastQC Report
Thu 28 Apr 2016
ERR1042162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800563
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.14875580374374722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37330.1332946268303909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.021.699251
TACGACG2300.016.0869565
CGACGGT2350.015.7446797
CTAGACT3350.014.9104484
TATACTG5250.014.7999995
ACGACGG2500.014.7999996
TTATACT7000.014.2714294
CAATACT4550.014.2307694
TAGTACT3250.014.2307694
CTACCCG2101.364242E-1014.09523910
GTATCAA20450.014.0220052
TCAACGC28400.013.9401413
TATAGCG800.00630160213.8755
ATCAACG28500.013.7614042
GTATAGA5650.013.7522131
CAACGCA28900.013.6989624
CGAACGA1652.598881E-713.45454516
CGTATTA1951.027729E-813.28205215
CGAACGT850.00940914213.0588244
CCTATCG850.00940914213.05882423
Produced by FastQC (version 0.11.5)