FastQCFastQC Report
Thu 28 Apr 2016
ERR1042161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4222903
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75000.17760294280972116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66920.15846918577102054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50440.11944389913763115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.019.5239851
TATACCG1606.311893E-1016.18755
GACGGAC7100.015.6338027
ACGGACC7250.015.3103458
CGACGGT5600.014.8660727
TCTATAC8600.014.6279073
TACGACG5750.014.478265
TCTAGCG3350.014.35820928
TTAATAC6750.014.2518513
TATACTG8850.014.2146895
ACGACGG5900.014.1101696
ATACCGT7250.013.779316
TAATACT9800.013.5918384
GACGGTA6200.013.4274198
TTAGACT4150.013.3734944
CTCTATA6950.013.3093532
TAAGGGT6400.013.2968754
TAGACTG6150.013.2357735
TAGACAG7550.013.2317895
GTATCAA40150.013.2241592
Produced by FastQC (version 0.11.5)