FastQCFastQC Report
Thu 28 Apr 2016
ERR1042160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280005
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59350.18094484612066142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51380.15664610267362397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.021.1846961
TTTAGAC4150.016.0481933
TATACTG6350.015.7322835
ACCGTCC1954.1836756E-1115.1794878
GTATCAA26850.014.7448792
TAGACAG7850.014.6114655
TCAACGC38050.014.391593
CGACGGT3350.014.3582097
ACGACGG3350.014.3582096
ATCAACG38200.014.2866492
TACGACG3500.014.2714295
GACGGAC5150.014.0097097
CAACGCA39550.013.8457654
ACGGACC5450.013.5779828
TACGAAC1102.4585382E-413.45454528
GTAAACG2355.638867E-1113.38297927
AACGCAG41250.013.3648495
CTACACT8100.013.2469144
CTAGACA5600.013.2142854
AGCGTTA1703.7373138E-713.05882410
Produced by FastQC (version 0.11.5)