FastQCFastQC Report
Thu 28 Apr 2016
ERR1042159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6133387
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88470.1442433030884893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87900.14331396339412464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63670.1038088742810457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.023.500831
TATACTG13000.015.5115395
GTATCAA48250.014.8766842
TACCGTC4550.014.63736257
ATACCGT6200.014.6209686
GACGGAC6050.014.3719017
TAACGCT3350.014.3582094
ACGGACC6650.013.6315788
CTAGTAC4550.013.4175823
ATAACGC5550.013.3333333
TTAGCGA1951.0286385E-813.28205227
CTACACT15050.013.2757484
ACCGTCG4350.013.1839088
TAGACAG11850.012.9578065
CGTCGTA3450.012.86956510
TCTATAC11650.012.3862663
CTAGACA9400.012.2021284
TTATACT16150.012.1424154
GTATTAG13600.012.1066181
TACGACG4750.012.0736845
Produced by FastQC (version 0.11.5)