FastQCFastQC Report
Thu 28 Apr 2016
ERR1042158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10437617
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT225700.2162370970308644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221020.21175331495685268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT177680.17023042711760739No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT165820.1588676802377401No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT135470.12979016187315554No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG116750.11185503357710863No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC114410.10961314254010278No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA113030.10829100167212496No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC112380.10766825416184556No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA110010.10539762093205757No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG109150.10457367807230329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92550.021.6882761
AAGACGG37600.018.4999985
ACGGACC38900.017.3110548
GACGGAC39200.017.1785727
CGCAAGA39900.016.8771932
CAAGACG43600.016.8027524
CGGACCA41600.016.2764429
AGACGGA42000.016.2095246
TACCGTC27250.016.0899077
CGAACGA18100.015.84254216
ACCGTCG28600.015.459798
TCTAGCG21950.015.25512528
GCGCAAG46300.015.1835851
CGTCGTA28900.015.10726610
CTAGCGG22100.015.06787429
CCGTCGT29150.014.8507729
ATACCGT31450.014.8235296
ACGAACG19800.014.76262715
TAAACGC26450.014.6880928
ATAACGA19650.014.68702312
Produced by FastQC (version 0.11.5)