FastQCFastQC Report
Thu 28 Apr 2016
ERR1042157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5756475
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT84170.14621795456420814No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT78030.1355517048193556No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA74820.12997537555535288No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC74430.12929787760738992No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG64780.11253414633087089No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA63710.11067536990953665No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC61680.10714890623167825No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC59050.10258013801849222No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG58190.10108616818452264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.020.8751
TCTAGCG14500.017.8620728
TCGTTTA12450.017.68273230
CTAGCGG14750.017.55932229
AAGACGG19650.017.2290085
CGAACGA10900.017.14220216
GACGGAC18700.016.719257
CGCAATA15700.016.49681536
TTAGACT4950.016.4444454
ACGAACG11350.016.29955915
ACGGACC19300.016.2953388
CGCAAGA20050.015.9625932
AGACGGA19800.015.8838396
CCGGTCG14350.015.72822420
TAACGCC15000.015.6633354
TAACGAA12200.015.46721313
CGTTTAT14500.015.43793231
TAAACGC12700.015.29527628
ATAACGA12400.015.21774112
GCGCAAG21950.015.086561
Produced by FastQC (version 0.11.5)