FastQCFastQC Report
Thu 28 Apr 2016
ERR1042156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5626790
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT91120.16193957833862646No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT88540.15735437078689626No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC74570.13252671594283774No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA72100.12813700173633635No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC67150.11933980120104003No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG66020.11733155138187137No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC65060.11562542764169269No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA61950.1100982976084055No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG57660.10247405714448202No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC57390.10199420984255676No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG56580.100554667936781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG902.1552496E-618.55
AAGACGG20350.018.3636365
ACGGACC19900.018.1281418
CAAGACG21550.017.856154
GACGGAC19700.017.8426387
CGGACCA20550.017.644779
CGCAAGA20900.017.1722492
GCGCAAG23450.016.4882741
AGACGGA21850.016.4256296
TATACTG7850.016.2611475
TCTAGCG12950.015.57142928
TCGTTTA12750.015.5254930
TTAGAGT10700.015.387854
GGTATCA32500.015.1984621
CGCAATA13650.015.17948736
GTTCAAA26150.015.0688331
CGAACGA10100.015.01980116
GAGCGAA23550.014.9256916
CTAGCGG13650.014.90842529
CGAAAGC23750.014.72210519
Produced by FastQC (version 0.11.5)