FastQCFastQC Report
Thu 28 Apr 2016
ERR1042155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612220
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT157040.16337537010180792No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT142770.14852968408962758No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC121700.1266096697745162No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA120790.1256629581928004No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG116060.12074213865267337No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC114270.11887992576116652No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA112590.11713215053338355No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC112240.1167680306942621No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG103050.10720728406133026No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG103050.10720728406133026No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC100830.10489772393890277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.021.1685561
AAGACGG36400.018.6016485
GACGGAC35600.018.1882027
TCTAGCG22450.018.12917528
ACGGACC36350.018.06748
CAAGACG38650.017.7580834
CGCAAGA38700.017.2093032
CTAGCGG23950.016.99373829
AGACGGA39100.016.8439886
CGGACCA39500.016.7202539
GTAAACG22350.015.89261727
CGAACGA17200.015.5959316
TAAACGC22750.015.53186828
GCGCAAG44100.015.5215411
GTTCAAA42000.015.4166671
ACGAACG17500.015.11714315
GCAAGAC50200.015.1095633
CGCAATA28150.014.91829536
TATACTG11700.014.8632475
TTAACGG3150.014.68253935
Produced by FastQC (version 0.11.5)