FastQCFastQC Report
Thu 28 Apr 2016
ERR1042154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7799818
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT134100.17192708855514321No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT120240.15415744316085322No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA96810.12411828070860115No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC95250.12211823404084557No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC91260.11700273006370147No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG90580.11613091484955161No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC87580.11228467125771396No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA85890.11011795403431208No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG84700.10859227740954983No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA82400.10564349065580762No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG82210.10539989522832455No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG80100.10269470390206542No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC79900.10243828766260957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG29700.018.8737375
GTATACG1552.1827873E-1117.9032251
CAAGACG31600.017.8560124
GACGGAC31300.017.081477
ACGGACC31750.017.0141758
TCGTTTA17900.016.84636930
GGTATCA44300.016.5372471
CGCAAGA32300.016.438082
AGACGGA33350.016.419796
TATACTG11100.016.3333345
CTAGCGG18350.016.33242629
CGAACGA13700.016.2043816
TCTAGCG18650.016.168928
ACGAACG13900.016.10431715
CGGACCA34250.015.6102199
CGAGCCG35050.015.4650515
TAAACGC19550.015.42455228
TTCGGGC19550.015.42455235
GTAAACG19650.015.15776127
ATAACGA15300.015.11437912
Produced by FastQC (version 0.11.5)