FastQCFastQC Report
Thu 28 Apr 2016
ERR1042153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16023046
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT286150.17858651844349693No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT261290.16307136608108097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC226380.14128399806129247No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA217320.13562964245375067No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG209320.13063683397027007No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC191770.11968386035963449No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC187930.11728731228756381No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA182020.11359887502039251No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC178670.111508136467935No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG173680.10839387217636398No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG172970.10795076042345506No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA171430.10698964479038506No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG167580.10458685570771001No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA166470.10389410353062707No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT166030.10361949906403564No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG163140.10181584699937828No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG161660.10089217742993437No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC160780.10034296849675149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.019.4487171
AAGACGG64050.018.2833715
GACGGAC62800.018.2054147
ACGGACC63900.017.892028
AGACGGA66900.017.3662196
TCTAGCG39250.017.20382328
CGCAAGA67450.017.1423262
CAAGACG70600.017.058784
CTAGCGG40000.017.0229
TCGTTTA39450.016.83523430
CGGACCA68950.016.5547499
CGAACGA29950.016.55425616
ACGAACG30800.016.21753115
GCGCAAG73450.015.9938731
TAAACGC40000.015.632528
GTAAACG40200.015.462687527
ATAACGA33150.015.1236812
TAACGAA33250.015.07819613
TTCGGGC40850.015.03549635
AACGAAC33150.015.01206714
Produced by FastQC (version 0.11.5)