FastQCFastQC Report
Thu 28 Apr 2016
ERR1042152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613974
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT732372.8017493670556783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT525862.011726206917131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT444401.7000934209751128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297681.1388024517458857No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA154180.5898298911924909No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT88830.3398274045571991No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA78550.3005003110206911No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76610.29307866107313996No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA62460.2389465235690944No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA61060.2335906937100369No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.2215783324547222No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56460.2159929670302765No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA47790.18282507783168464No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47250.18075925774319102No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA40960.1566962793049969No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.138027386653425No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.13075876041613266No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA33880.1296110825891918No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA32300.12356664603396975No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA31250.1195497736396766No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT31140.11912895843646494No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA30240.11568592495564226No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA27620.10566287193369177No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA26550.10156948768426924No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA26430.10111041655349287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTC502.7033794E-422.19999928
AAGACGG6950.021.0287765
CGGTTAC609.241111E-418.531
GAACCGT902.1543528E-618.56
GACGGAC7350.018.122457
ACGGACC7750.017.9032278
AGACGGA7650.017.6535956
GTGCTAC953.6099464E-617.5263161
CGCAAGA8000.017.3437482
CGGACCA7800.017.3141029
CGAACGA2700.017.1296316
CTAGATA3900.017.0769233
CGTTATA650.001580662117.0769232
CAAGACG8450.016.8579884
GCGCAAG8650.016.6820811
TCTAGCG3850.016.33766428
CGCCACA3550.016.154931
TTAACGG1151.2436012E-616.08695635
CTAGCGG4150.015.60240929
GAACAGT53600.015.1865681
Produced by FastQC (version 0.11.5)