FastQCFastQC Report
Thu 28 Apr 2016
ERR1042151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281246
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT935824.102231850488724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT625952.743895222172444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT521692.2868642838168265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323901.419838106017501No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA159720.7001436934026405No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA99210.43489391323864246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT97360.4267843099779681No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75670.33170469120822565No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA70940.3109704082768803No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.2548607208516749No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.2425428910341103No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA52600.23057574676295323No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA50080.21952915205111592No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA49880.2186524381850971No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49470.2168551747597585No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47010.20607159420772686No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG45190.19809349802695544No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41890.18362771923764468No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA39730.1741592094846413No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.16359480739911433No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA36830.1614468584273682No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA35480.15552903983174107No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA34480.1511454705016469No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG33760.1479893005839791No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30550.13391804303437685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30240.13255913654204762No Hit
GGTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCC28380.12440569758807249No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA28100.12317829817564611No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT26280.11520020199487474No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA25350.11112348251788716No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24830.10884402646623818No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC24830.10884402646623818No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24820.10880019077293725No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA24160.10590703501507509No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA24080.10555634946866756No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA23980.10511799253565815No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23890.10472347129594968No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23800.10432895005624121No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA23420.10266319371080541No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.10253168663090259No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA23220.10178647984478659No Hit
GTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCT23020.10090976597876775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1100.025.2272722
CGCCACA5150.022.990291
CCACAAC14700.020.0102041
TTCGGGC2600.019.92307935
ACAACAC15050.019.4219263
CACAACA15350.019.4039082
CGAACGA2450.018.8775516
CAGCGTA801.6178083E-518.54
ACGCTTA500.00703695518.49999825
ACGAACG2550.018.13725515
CAAGACG5750.017.6956524
CGAGCCG4850.017.54639215
AAGACGG6050.017.4297525
GTATTAG4050.017.3580251
GCGCAAG6200.017.3064521
GCCACAA6750.017.2666682
TAGGACT1502.5102054E-1017.2666664
GTTATAC2050.017.1463413
ACGGACC5950.017.1008428
GTCCTAC1301.3951649E-817.0769251
Produced by FastQC (version 0.11.5)