FastQCFastQC Report
Thu 28 Apr 2016
ERR1042150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958970
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT660262.2313845696306482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT424361.4341476932851633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT370101.250773073062586No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186400.6299489349334396No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA73310.24775513100842522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60340.2039223108040974No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA48180.16282692964105752No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45470.15366833729304455No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG41550.14042048415495934No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.13132948289438554No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC37710.12744299536663095No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA37210.1257532181806507No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA36670.12392825881979203No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG31870.10770639783438156No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT31860.10767260229066196No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG31470.10635457608559734No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.1060166206484013No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC31270.10567866521120527No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC30550.10324538606339367No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA30530.10317779497595447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCACA6200.018.51
AAGACGG9550.017.6282715
CTAGCGG5450.017.31192629
GTACCGT650.001580728817.0769236
ACAACAC17450.016.8567313
CGGTCCA5550.016.66666610
TCTAGCG5650.016.37168128
CGAACGT3200.016.18754
CGTTATA803.3848168E-416.18752
CCGGTCC6200.016.1129049
TTAGACT1504.6802597E-916.0333334
CAAGACG11600.015.9482764
CCACAAC18100.015.9447511
ATTAGAC1403.478999E-815.8571433
GTCTAAC1403.478999E-815.8571431
TCGAACG3300.015.696973
ACGGACC10400.015.6538468
CGAGACG1902.7284841E-1115.57894823
CGCAAGA10500.015.5047622
CGAGCCG10900.015.44495415
Produced by FastQC (version 0.11.5)