FastQCFastQC Report
Thu 28 Apr 2016
ERR1042148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806181
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT653183.616359600726616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT412282.282606228279447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293071.6225948562187291No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179520.9939203213852875No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA80020.443034225252065No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.34254595746494954No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA42180.23353141241104852No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA42050.2328116617326835No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA34190.18929442840999877No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.18220765249994325No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.1731277208651846No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA28670.15873270729788433No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG28640.1585666109874924No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC26630.14743815819123332No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA26210.14511280984574637No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.13990845878679933No Hit
GGTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCC25120.1390779772348397No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA24780.1371955523837312No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23070.1277280626913914No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22980.1272297737602156No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA22330.12363102036839055No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.11914641998780853No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA20600.11405279980245611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20450.11322231825049649No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.11205964407775301No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA19680.1089591796171037No Hit
GTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCT19600.10851625612272524No Hit
ACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTG18420.10198313458064281No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA18410.10192776914384549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACG2100.021.14285727
CGCCACA4500.019.7333341
ACAACAC10400.019.2115383
AAGACGG4500.018.9111125
GCTTCGG2450.018.8775533
CGAACTA609.2396047E-418.524
ACTAGAC801.6175038E-518.53
TTAGTAC609.2396047E-418.53
CGATCGG902.1536016E-618.536
GCGCAAG4800.017.7291661
TACCGTC3450.017.6956527
GCGATCG953.6086967E-617.52631635
AGCGAAC752.0680907E-417.26666622
GGTTATC2250.017.2666662
CGCAAGA5050.017.2178232
CCGTCGT3450.017.159429
GCCACAA5400.017.129632
CGCGGTC1301.3946192E-817.07692310
CACAACA11500.017.0521742
TTCGGGC2500.017.0235
Produced by FastQC (version 0.11.5)