FastQCFastQC Report
Thu 28 Apr 2016
ERR1042147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9514094
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1255341.3194530136027667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT944660.9929058930887166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT792080.832533292187359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354980.3731096203169739No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT147070.15458119291232564No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA135220.14212598698310105No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA133890.14072806091678305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT116030.12195591088336945No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG115280.12116760671063373No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT109810.11541824161081443No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG101590.10677842787763081No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC99050.10410870441263245No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA98880.10393002213347903No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA98690.1037303184097193No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC95150.10000952271440665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG35450.018.578285
ACGGACC34400.018.58
GACGGAC34600.018.179197
CGCAAGA36000.017.986112
CAAGACG37550.017.9826894
GCGCAAG37450.017.9813081
CGGACCA36150.017.6556029
AGACGGA37500.017.026
CGAACGA15600.016.48397416
TCTAGCG18200.016.46703128
CTAGCGG18600.016.11290429
CGCCACA20400.015.7794111
TCGTTTA22000.015.64090930
ACGAACG16500.015.58484915
ACAACAC52150.015.3604983
TACGACG24800.015.2923395
GCAAGAC47100.015.2399153
GCGAAAG42100.015.1163918
CGACGGT25150.015.0795237
CGAAAGC42300.015.00118319
Produced by FastQC (version 0.11.5)