FastQCFastQC Report
Thu 28 Apr 2016
ERR1042146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010280
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185800.9242493582983464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137630.6846309966770798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105680.5256979127285751No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47270.2351413733410271No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA23570.1172473486280518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT752.0682375E-417.26666614
TACGACG2550.016.6862745
ACGGACC4000.016.6500028
TATACCG1005.882197E-616.6500025
CGTCGTA2500.016.27999910
AAGACGG4550.015.8571425
CGGACCA4150.015.6024099
CCGCTAG2850.015.57894736
CGACGGT2750.015.4727287
AGTACCG855.366633E-415.2352945
ACGACGG2800.015.1964296
GACGGAC4300.015.0581397
TACCGTC2850.014.9298247
GGTATCA60650.014.8854081
ATTAGAC2251.8189894E-1214.83
CCGTCGT2750.014.89
CCGACGT1001.094011E-414.813
GACGGTA3000.014.7999998
TACGTAG750.00410582914.79999914
TATACTG3400.014.6911765
Produced by FastQC (version 0.11.5)