FastQCFastQC Report
Thu 28 Apr 2016
ERR1042145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962564
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.6243740675944665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46820.4864092153872366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36680.381065570704909No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT16930.17588440872503022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.13318594919402763No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10730.11147310724273919RNA PCR Primer, Index 27 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG250.005495905429.59999825
ACGACGG405.939141E-527.7500026
GGTATCA16000.025.7843741
TACGACG451.3229938E-424.6666665
GACGGTA551.9022418E-523.5454548
TATCGGC603.725476E-521.5833342
CATTGCG705.1005027E-621.14285729
CGTTCCC450.003825362820.55555527
ATGCACG450.003825362820.55555511
GCCGTCT3000.019.73333236
TCTATAC759.263209E-619.7333323
TATACAC3500.019.5571443
CCGTCTT3150.019.3809537
TTAGGAC1103.849709E-818.53
TAGGTCG801.616225E-518.521
ACCGTCC609.235328E-418.58
GTACTAG1054.795256E-717.6190471
TATGCCG3600.017.47222133
GTATCGG852.7223705E-517.4117641
GTATCAA23850.017.2976932
Produced by FastQC (version 0.11.5)