FastQCFastQC Report
Thu 28 Apr 2016
ERR1042143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4741042
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT398240.8399841216340206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT272230.5741986677190373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218270.4603840252838933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97740.20615721185342797No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA49970.1053987709874749No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47730.10067407122737998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACG7400.017.7527
ACGGACC10650.017.718318
GACGGAC10650.017.5446017
CGAACGA5850.016.76068516
CGGACCA11650.016.673829
TAAACGC8250.016.5939428
TAGAGTG6950.015.7050365
ACGCTTC8600.015.70348831
AGACGGA12700.015.5866156
CGCAAGA12500.015.3922
AAGACGG13100.015.1106875
CGCCACA10300.014.9077661
ATTAGAG8750.014.83
TTCGGGC9750.014.61025635
TTAGAGT6600.014.5757574
GCGAAAG13050.014.4597718
CTTCGGG10000.014.42999934
CTAGCGG6050.014.37190129
CTAGTAC3350.014.3582083
TTAACGG2450.014.3469435
Produced by FastQC (version 0.11.5)