FastQCFastQC Report
Thu 28 Apr 2016
ERR1042142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088113
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT645252.08946369514328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT445791.4435676414690783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT372691.2068535056845395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161580.5232321485645117No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA92750.3003452270043227No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58190.1884322238208252No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA56580.18321868403131622No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC56290.18227959922451023No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA53570.17347163138136462No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG49210.15935297704455761No Hit
GGTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCC48070.15566140228676864No Hit
GTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCT46050.14912019087384432No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA41000.1327671623415335No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA38050.12321440309988657No Hit
ACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTG37660.12195149594590614No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA36570.1184218323617044No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA36350.11770942319792055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCACA10150.020.4137941
ACAACAC24800.018.2762093
CCACAAC26550.017.2806031
GCCACAA13050.016.8697322
CACAACA27550.016.7876592
CGAACGA2800.016.51785716
CTAGTAC2050.016.2439043
ACGAACG2850.016.2280715
TAACGAA3000.016.03333313
CGGGTAA2350.015.74467924
TATACTG4350.015.7356335
TATAGAC2003.6379788E-1215.7253
CTAGCGG2650.015.35849129
TACGACG4100.015.3414645
TACGTCG855.3677906E-415.23529421
AACACAG30850.015.1717995
GCGGGTA2600.014.94230723
ACCCGTT2850.014.92982430
CGCAAGA6350.014.8582682
TCTAGCG2750.014.828
Produced by FastQC (version 0.11.5)